Molecular characterization of some strains of Newcastle disease virus isolated in Province of Vojvodina, Republic of Serbia

Abstract

Five strains of Newcastle disease virus (NDV) were obtained from poultry in Vojvodina, Serbia during the outbreaks in 2006 and 2007. These isolates were confirmed and genotypically characterized by reverse transcription polymerase chain reaction (RT-PCR) with primer specific to the viral fusion (F) protein (572bp), and by sequencing of partial F gene for phylogenetic analysis. Phylogenetic analysis showed that all five isolated strains of Newcastle disease virus belong to genotype VII. At the same time, all five isolates were clustered in NDV subtype VIId. The examined NDV isolates express high similarity to each other (99.7-100%) and group together with the strains of Newcastle disease virus isolated previously from wild birds in Serbia during the same 2006 - 2007 outbreak. The analysis of the isolates F gene cleavage sites has shown that all five isolated strains of Newcastle disease virus had a cleavage site motif 112R-R-Q-K-R-F-117 characteristic for highly virulent, velogenic strains. [Projekat Ministarstva nauke Republike Srbije, br. 31008 i br. 31084]

Keywords

• Newcastle disease virus
• phylogenetic analysis
• RT-PCR