International Journal of Hyperthermia (Jan 2021)

Multiple cylindrical interstitial laser ablations (CILAs) of porcine pancreas in ex vivo and in vivo models

  • Van Gia Truong,
  • Hyeonsoo Kim,
  • Jin-Seok Park,
  • Van Nam Tran,
  • Hyun Wook Kang

DOI
https://doi.org/10.1080/02656736.2021.1972171
Journal volume & issue
Vol. 38, no. 1
pp. 1313 – 1321

Abstract

Read online

Background The therapeutic capacity of multiple cylindrical interstitial laser ablations (CILAs) of pancreatic tissue was evaluated with 1064 nm laser light in ex vivo and in vivo porcine pancreatic models. Methods A diffusing applicator was sequentially employed to deliver 1064 nm laser light in a cylindrical distribution to ablate a large volume of pancreatic tissue. Ex vivo tissue was tested at various power levels (5, 7, and 10 W) under US imaging. An in vivo porcine model was used to evaluate the clinical feasibility of multiple CILAs on pancreatic tissue at 5 W via laparotomy (N = 3). Results Multiple CILAs symmetrically ablated a range of ex vivo tissue volumes (2.4–6.0 cm3) at various power levels. Multiple CILAs warranted a therapeutic capacity of symmetrically ablating in vivo pancreatic tissue. Both ex vivo and in vivo pancreatic tissues after multiple CILAs at 5 W confirmed the absence of or minimal thermal injury to the peripheral tissue and carbonization. Conclusions The current findings suggest that the collective thermal effects from multiple CILAs can help widely ablate pancreatic tissue with minimal thermal injury. Further in vivo studies will investigate the safety of the proposed CILA treatment as well as acute/chronic responses of pancreatic tissue for clinical translations.

Keywords