Protective Effects of Imatinib and Ginkgo Biloba on Cisplatin-induced Ovarian Damage in Rats

Abstract

Introduction:In our study we aimed to observe the protective effects of imatinib and ginkgo biloba (GB) on cisplatin (CP)- induced ovarian damage in rats.Methods:Thirty-two female rats were included and assigned to four groups. Group 1 had no medication. Their ovaries were removed for examination and the serum Anti-Mullerian hormone (AMH) levels were measured. Group 2 received a single dose of 7.5 mg/kg intramuscular CP. Group 3 received a single dose of 7.5 mg/kg oral imatinib and 30 minutes later, a single dose of 7.5 mg/kg intramuscular CP was administered. Group 4 received 80 mg/kg oral GB for 10 days. Sixty minutes after the first administration of the GB, a single dose of 7.5 mg/kg intramuscular CP was administered. The ovaries and serum AMH levels of the rats were assessed after 10 days of observation.Results:Comparing group 1 and 2 showed that the total histopathological ovarian damage scores increased in the latter (p=0.044). This group also had decreased primordial follicles, preantral follicles and serum AMH (p=0.001, p=0.004 and p<0.001 respectively). In group 3, total histopathological ovarian damage score increased (p=0.020), and a reduction in primordial follicles (p=0.008) and serum AMH levels (p<0.001) was observed. In group 4, total histopathological ovarian damage score increased (p=0.016) as in groups 2 and 3. There was also a reduction in primordial follicles, preantral follicles and serum AMH levels (p<0.00, p=0.010 and p<0.001 respectively).Conclusion:It was concluded that imatinib and GB were not effective in preventing CP-induced ovarian damage in rats.

Keywords

• cisplatin
• imatinib
• ginkgo biloba
• anti-mullerian hormone
• ovary
• rat