Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam
Molong Han,
Daniel Smith,
Soon Hock Ng,
Tomas Katkus,
Aravind Simon John Francis Rajeswary,
Periyasamy Angamuthu Praveen,
Keith R. Bambery,
Mark J. Tobin,
Jitraporn Vongsvivut,
Saulius Juodkazis,
Vijayakumar Anand
Affiliations
Molong Han
Optical Sciences Centre and ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Science, Swinburne University of Technology, Hawthorn, VIC 3122, Australia
Daniel Smith
Optical Sciences Centre and ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Science, Swinburne University of Technology, Hawthorn, VIC 3122, Australia
Soon Hock Ng
Optical Sciences Centre and ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Science, Swinburne University of Technology, Hawthorn, VIC 3122, Australia
Tomas Katkus
Optical Sciences Centre and ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Science, Swinburne University of Technology, Hawthorn, VIC 3122, Australia
Aravind Simon John Francis Rajeswary
Institute of Physics, University of Tartu, W. Ostwaldi 1, 50411 Tartu, Estonia
Periyasamy Angamuthu Praveen
Institute of Physics, University of Tartu, W. Ostwaldi 1, 50411 Tartu, Estonia
Keith R. Bambery
Infrared Microspectroscopy (IRM) Beamline, ANSTO—Australian Synchrotron, Clayton, VIC 3168, Australia
Mark J. Tobin
Infrared Microspectroscopy (IRM) Beamline, ANSTO—Australian Synchrotron, Clayton, VIC 3168, Australia
Jitraporn Vongsvivut
Infrared Microspectroscopy (IRM) Beamline, ANSTO—Australian Synchrotron, Clayton, VIC 3168, Australia
Saulius Juodkazis
Optical Sciences Centre and ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Science, Swinburne University of Technology, Hawthorn, VIC 3122, Australia
Vijayakumar Anand
Optical Sciences Centre and ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Science, Swinburne University of Technology, Hawthorn, VIC 3122, Australia
Phase imaging of biochemical samples has been demonstrated for the first time at the Infrared Microspectroscopy (IRM) beamline of the Australian Synchrotron using the usually discarded near-IR (NIR) region of the synchrotron-IR beam. The synchrotron-IR beam at the Australian Synchrotron IRM beamline has a unique fork shaped intensity distribution as a result of the gold coated extraction mirror shape, which includes a central slit for rejection of the intense X-ray beam. The resulting beam configuration makes any imaging task challenging. For intensity imaging, the fork shaped beam is usually tightly focused to a point on the sample plane followed by a pixel-by-pixel scanning approach to record the image. In this study, a pinhole was aligned with one of the lobes of the fork shaped beam and the Airy diffraction pattern was used to illuminate biochemical samples. The diffracted light from the samples was captured using a NIR sensitive lensless camera. A rapid phase-retrieval algorithm was applied to the recorded intensity distributions to reconstruct the phase information. The preliminary results are promising to develop multimodal imaging capabilities at the IRM beamline of the Australian Synchrotron.
