Whole transcriptome sequencing identifies a competitive endogenous RNA network that regulates the immunity of bladder cancer
Sanhe Liu,
Yiqi Wang,
Liqun Duan,
Diansheng Cui,
Kangli Deng,
Zhiqiang Dong,
Shaozhong Wei
Affiliations
Sanhe Liu
College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, 430070, China; Department of Urology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430079, China; Division of Infection and Immunity, Systems Immunity Research Institute, School of Medicine, Cardiff University, Cardiff, CF14 4XN, United Kingdom
Yiqi Wang
College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, 430070, China; Center for Neurological Disease Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, China
Liqun Duan
Department of Radiation Oncology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430079, China; College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, 430070, China
Diansheng Cui
Department of Urology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430079, China
Kangli Deng
Department of Urology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430079, China
Zhiqiang Dong
College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, 430070, China; Center for Neurological Disease Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, China; Corresponding author. College of Biomedicine and Health, Huazhong Agricultural University, Shizishan Road, Hongshan District, Wuhan, Hubei Province, 430070, China.
Shaozhong Wei
College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, 430070, China; Department of Urology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430079, China; Corresponding author. Department of Urology, Hubei Cancer Hospital, 116 Zhuodaoquan South Road, Hongshan District, Wuhan, Hubei Province, 430079, China.
Several types of non-coding RNAs such as circRNAs, lncRNAs, and miRNAs have been identified to regulate mRNAs through the mechanism known as the competitive endogenous RNA (ceRNA) network. To explore the role of the ceRNA regulatory network in the immune microenvironment of bladder cancer, whole-transcriptome sequencing of bladder tumor and its peritumoral tissues from 38 bladder cancer patients, with a total of 63 samples, was performed to screen differentially expressed circ-, lnc-, mi-, and mRNAs to construct a circ/lnc-mi-mRNA regulatory network with pruning algorithms. We excavated a key immune-related gene BDNF to build the final ceRNA network as hsa-miR-107 sponged by hsa-circ-000211, AC108488.1, and LINC00163. Finally, a meta-analysis of 7 public datasets demonstrated that low expression of BDNF and high expression of hsa-miR-107 were associated with longer survival. Our study identified a ceRNA regulatory network as a potentially new prognostic marker and molecular therapeutic target of bladder cancer.
