Simultaneous Determination of Tetrodotoxin in the Fresh and Heat-Processed Aquatic Products by High-Performance Liquid Chromatography–Tandem Mass Spectrometry
Hongli Ye,
Yinfeng Xi,
Liangliang Tian,
Dongmei Huang,
Xuanyun Huang,
Xiaosheng Shen,
Youqiong Cai,
Yuan Wangs
Affiliations
Hongli Ye
Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
Yinfeng Xi
Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
Liangliang Tian
Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
Dongmei Huang
Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
Xuanyun Huang
Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
Xiaosheng Shen
Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
Youqiong Cai
Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
Yuan Wangs
Laboratory of Aquatic Product Quality, Safety and Processing, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
Tetrodotoxin (TTX) was simultaneously detected in the fresh and heat-processed aquatic products by high-performance liquid chromatography–tandem mass spectrometry method. The detection conditions were investigated, including the chromatography column and mobile phase. Based on the optimized parameters, a sensitive determination method of TTX was established. The proposed method featured the merits of a good linear relationship between signal and TTX concentration (R2 = 0.9998), a wide detection matrix-based range of 0.2–100 ng/g, and a low detection limit of 0.2 ng/g, etc. The spiked assays evidenced its accuracy and reliability with recoveries of 90.5–107.2%. Finally, the developed method was simultaneously successfully applied in the determination of TTX in various fresh and heat-processed aquatic products.
