Generation of two non-integrated induced pluripotent stem cell lines from urine-derived cells of a Chinese patient carrying NF1 gene mutation

Liang Shi; Yazhou Cui; Zijuan Qi; Xiaoyan Zhou; Jing Luan; Jinxiang Han

Stem Cell Research (Jul 2020)

Generation of two non-integrated induced pluripotent stem cell lines from urine-derived cells of a Chinese patient carrying NF1 gene mutation

Liang Shi,
Yazhou Cui,
Zijuan Qi,
Xiaoyan Zhou,
Jing Luan,
Jinxiang Han

Affiliations

Liang Shi: Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
Yazhou Cui: Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
Zijuan Qi: Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
Xiaoyan Zhou: Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
Jing Luan: Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
Jinxiang Han: Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China; Corresponding author at: Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji'nan, Shandong, China.

Journal volume & issue: Vol. 46
p. 101842

Abstract

Read online

Mutations in the neurofibromin (NF1) gene cause neurofibromatosis type 1 (NF1), a complex tumour predisposition syndrome. Here, we generated two induced pluripotent stem cell (iPSC) lines using urine cells (UCs) derived from a 21-year-old female NF1 patient carrying c.496_497delGT mutation in the NF1 gene (p.Val166LeufsTer7). The newly derived SMBCi003-A and SMBCi003-B iPSC lines used as a cellular model to unravel pathogenesis of NF1.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal