Bioassay-Guided Chemical Study of the Anti-Inflammatory Effect of Senna villosa (Miller) H.S. Irwin & Barneby (Leguminosae) in TPA-Induced Ear Edema
Ana del Carmen Susunaga-Notario,
Salud Pérez-Gutiérrez,
Miguel Ángel Zavala-Sánchez,
Julio Cesar Almanza-Pérez,
Atilano Gutiérrez-Carrillo,
Daniel Arrieta-Báez,
Ana Laura López-López,
Rubén Román-Ramos,
José Luis Eduardo Flores-Sáenz,
Francisco Javier Alarcón-Aguilar
Affiliations
Ana del Carmen Susunaga-Notario
Doctorado en Biología Experimental, D.C.B.S., Universidad Autónoma Metropolitana, Unidad Iztapalapa, Av. San Rafael Atlixco No.186 Colonia, Vicentina, Iztapalapa 09340, Mexico
Salud Pérez-Gutiérrez
Laboratory of Productos Naturales, Departamento Sistemas Biológicos, D.C.B.S., Universidad Autónoma Metropolitana Unidad Xochimilco, Calzada del Hueso 1100, Col, Villa Quietud, Coyoacán 04690, Mexico
Miguel Ángel Zavala-Sánchez
Laboratory of Productos Naturales, Departamento Sistemas Biológicos, D.C.B.S., Universidad Autónoma Metropolitana Unidad Xochimilco, Calzada del Hueso 1100, Col, Villa Quietud, Coyoacán 04690, Mexico
Julio Cesar Almanza-Pérez
Laboratory of Farmacología, Departamento Ciencias de la Salud, D.C.B.S., Universidad Autónoma Metropolitana Unidad Iztapalapa, Av. San Rafael Atlixco No.186 Colonia, Vicentina, Iztapalapa 09340, Mexico
Atilano Gutiérrez-Carrillo
Laboratory of RMN, Departamento de Química, D.C.B.I. Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, México D.F. 09340, Mexico
Daniel Arrieta-Báez
Instituto Politécnico Nacional-CNMN, Calle, Luis Enrique Erro s/n, Unidad Profesional Adolfo López Mateos, Gustavo A, Madero 07738, Mexico
Ana Laura López-López
Doctorado en Biología Experimental, D.C.B.S., Universidad Autónoma Metropolitana, Unidad Iztapalapa, Av. San Rafael Atlixco No.186 Colonia, Vicentina, Iztapalapa 09340, Mexico
Rubén Román-Ramos
Laboratory of Farmacología, Departamento Ciencias de la Salud, D.C.B.S., Universidad Autónoma Metropolitana Unidad Iztapalapa, Av. San Rafael Atlixco No.186 Colonia, Vicentina, Iztapalapa 09340, Mexico
José Luis Eduardo Flores-Sáenz
Laboratory of Farmacología, Departamento Ciencias de la Salud, D.C.B.S., Universidad Autónoma Metropolitana Unidad Iztapalapa, Av. San Rafael Atlixco No.186 Colonia, Vicentina, Iztapalapa 09340, Mexico
Francisco Javier Alarcón-Aguilar
Laboratory of Farmacología, Departamento Ciencias de la Salud, D.C.B.S., Universidad Autónoma Metropolitana Unidad Iztapalapa, Av. San Rafael Atlixco No.186 Colonia, Vicentina, Iztapalapa 09340, Mexico
Senna villosa (Miller) is a plant that grows in México. In traditional Mexican medicine, it is used topically to treat skin infections, pustules and eruptions and to heal wounds by scar formation. However, studies of its potential anti-inflammatory effects have not been performed. The aim of the present study was to determine the anti-inflammatory effect of extracts from the leaves of Senna villosa and to perform a bioassay-guided chemical study of the extract with major activity in a model of ear edema induced by 12-O-tetradecanoylphorbol 13-acetate (TPA). The results reveal that the chloroform extract from Senna villosa leaves has anti-inflammatory and anti-proliferative properties. Nine fractions were obtained from the bioassay-guided chemical study, including a white precipitate from fractions 2 and 3. Although none of the nine fractions presented anti-inflammatory activity, the white precipitate exhibited pharmacological activity. It was chemically characterized using mass spectrometry and infrared and nuclear magnetic resonance spectroscopy, resulting in a mixture of three aliphatic esters, which were identified as the principal constituents: hexyl tetradecanoate (C20H40O2), heptyl tetradecanoate (C21H42O2) and octyl tetradecanoate (C22H44O2). This research provides, for the first time, evidence of the anti-inflammatory and anti-proliferative properties of compounds isolated from Senna villosa.
