Memorias do Instituto Oswaldo Cruz (Sep 2007)

Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags

  • Luciana Girotto Gentil,
  • Fernanda Lasakosvitsch,
  • José Franco da Silveira,
  • Márcia Regina Machado dos Santos,
  • Clara Lúcia Barbiéri

DOI
https://doi.org/10.1590/s0074-02762007000600008
Journal volume & issue
Vol. 102, no. 6
pp. 707 – 711

Abstract

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The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis ESTs was also determined in L. (L.) amazonensis and L. (L.) major. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.

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