Infection and Drug Resistance (Apr 2021)

Direct-on-Target Microdroplet Growth Assay Applications for Clinical Antimicrobial Susceptibility Testing

  • Li R,
  • Tang H,
  • Xu H,
  • Ren Y,
  • Li S,
  • Shen J

Journal volume & issue
Vol. Volume 14
pp. 1423 – 1425

Abstract

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Rongrong Li,1 Hao Tang,2 Huaming Xu,2 Yingli Ren,3 Shujin Li,1 Jilu Shen2 1Department of Clinical Laboratory, Hefei Hospital Affiliated to Anhui Medical University the Second People’s Hospital of Hefei City, Hefei, Anhui Province, People’s Republic of China; 2The Fourth Affiliated Hospital of Anhui Medical University Laboratory, Hefei, People’s Republic of China; 3The Second Affiliated Hospital of Anhui Medical University Laboratory, Hefei, People’s Republic of ChinaCorrespondence: Jilu ShenThe Fourth Affiliated Hospital of Anhui Medical University Laboratory, No.100 Huaihai Avenue, Xinzhan District, Hefei, Anhui Province, 230012, People’s Republic of ChinaTel +86 151 5515 2963Email [email protected]: Direct-on-target microdroplet growth assay is a new technique for analysing bacterial sensitivity and mechanisms of resistance. It is based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and allows for easy and rapid testing. Here, we describe the development and procedure of the direct-on-target microdroplet growth assay and summarise the latest clinical applications.Keywords: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF MS, direct-on-target microdroplet growth assay, DOT-MGA, antimicrobial susceptibility testing

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