Amomum villosum Lour. fruit extract ameliorates high-fat diet-induced body mass gain and adipogenic pathways in C57BL/6 mice

Abstract

Amomum villosum Lour. is commonly used in Asian countries as an herbal remedy to medicate several diseases including type 2 diabetes. In this animal experiment, we examined the influence of Amomum villosum water extract (AVE) against metabolic variations in high-fat diet (HFD)-induced obesity in C57BL/6 mice. Mice were nourishing with a normal diet (control), high-fat diet (HFD), HFD + AVE 100 mg/kg body weight (b.wt.)/day, HFD + AVE 200 mg/kg b.wt./day, and HFD + AVE 500 mg/kg b.wt./day for 7 weeks. The AVE (100, 200, and 500 mg/kg)-treated animals exhibited substantial decreases in body mass, fat mass, adipocyte hypertrophy, and epididymal white adipose tissue (eWAT) collate to the HFD-fed group. AVE treatment also reduced hepatic triglyceride level and significantly increased the adiponectin expression in adipocytes. Furthermore, AVE treatment significantly inhibited adipogenesis in the AVE group by reducing the mRNA expression of sterol regulatory element-binding protein1 (SREBP1), fatty acid synthase (FAS), CCAAT/enhancer binding protein (C/EBP)-α, peroxisome proliferator-activated receptor (PPAR)-γ, and tumor necrosis factor (TNF)-α agreeing to the HFD-fed animals. These research outcomes recommend that AVE is possibly valuable for the prevention of HFD-induced obesity via modification of various pathways related with adipogenesis and food consumption.

Keywords

• Amomum villosum
• Adipogenesis
• HFD-induced obesity
• FAS
• SREBP1
• PPAR-γ