Optimizing Antioxidant Potential: Factorial Design-Based Formulation of Fucoidan and Gallic Acid-Conjugated Dextran Blends
Cynthia Haynara Ferreira Silva,
Maylla Maria Correia Leite Silva,
Weslley Souza Paiva,
Mayara Jane Campos de Medeiros,
Moacir Fernandes Queiroz,
Luciana Duarte Martins Matta,
Everaldo Silvino dos Santos,
Hugo Alexandre Oliveira Rocha
Affiliations
Cynthia Haynara Ferreira Silva
Departamento de Bioquimica, Programa de Pós-Graduação em Bioquímica e Biologia Molecular—PPgBBM, Centro de Biociências, Universidade Federal do Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
Maylla Maria Correia Leite Silva
Departamento de Bioquimica, Programa de Pós-Graduação em Bioquímica e Biologia Molecular—PPgBBM, Centro de Biociências, Universidade Federal do Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
Weslley Souza Paiva
Departamento de Bioquimica, Programa de Pós-Graduação em Bioquímica e Biologia Molecular—PPgBBM, Centro de Biociências, Universidade Federal do Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
Mayara Jane Campos de Medeiros
Laboratório de Química de Coordenação e Polímeros (LQCPol), Instituto de Química, Universidade Federal do Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
Moacir Fernandes Queiroz
Departamento de Bioquimica, Programa de Pós-Graduação em Bioquímica e Biologia Molecular—PPgBBM, Centro de Biociências, Universidade Federal do Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
Luciana Duarte Martins Matta
Departamento de Bioquimica, Programa de Pós-Graduação em Bioquímica e Biologia Molecular—PPgBBM, Centro de Biociências, Universidade Federal do Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
Everaldo Silvino dos Santos
Laboratório de Engenharia Bioquímica, Departamento de Engenharia Química, Universidade Federal do Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
Hugo Alexandre Oliveira Rocha
Departamento de Bioquimica, Programa de Pós-Graduação em Bioquímica e Biologia Molecular—PPgBBM, Centro de Biociências, Universidade Federal do Rio Grande do Norte—UFRN, Natal 59078-970, Brazil
The role of oxidative stress in health and homeostasis has generated interest in the scientific community due to its association with cardiovascular and neurodegenerative diseases, cancer, and other diseases. Therefore, extensive research seeks to identify new exogenous antioxidant compounds for supplementation. Polysaccharides are recognized for their antioxidant properties. However, polysaccharide chemical modifications are often necessary to enhance these properties. Therefore, dextran was conjugated with gallic acid (Dex-Gal) and later combined with fucoidan A (FucA) to formulate blends aimed at achieving superior antioxidant activity compared to individual polysaccharides. A factorial design was employed to combine FucA and Dex-Gal in different proportions, resulting in five blends (BLD1, BLD2, BLD3, BLD4, and BLD5). An analysis of surface graphs from in vitro antioxidant tests, including total antioxidant capacity (TAC), reducing power, and hydroxyl radical scavenging, guided the selection of BLD4 as the optimal formulation. Tests on 3T3 fibroblasts under various conditions of oxidative stress induced by hydrogen peroxide revealed that BLD4 provided enhanced protection compared to its isolated components. The BLD4 formulation, resulting from the combination of Dex-Gal and FucA, showed promise as an antioxidant strategy, outperforming its individual components and suggesting its potential as a supplement to mitigate oxidative stress in adverse health conditions.
