Resveratrol inhibits the proliferation of A549 cells by inhibiting the expression of COX-2

Abstract

Xia Li,1 Fang Li,1 Fangfang Wang,2 Jinfeng Li,2 Cunzhi Lin,2 Jianxin Du2 1Respiratory Department of Internal Medicine, Rizhao People’s Hospital of Shandong Province, Rizhao, Shandong, 276800, China; 2Pulmonary Department, Affiliated Hospital of QingDao University, Qingdao, Shandong, 266003, China Purpose: The aim was to investigate resveratrol effects on A549 cells proliferation. Methods: A total of 104 lung adenocarcinoma tissues and nontumor tissues were collected. BEAS-2B cells were cultured in RPMI 1640 medium (group A). A549 cells were treated with RPMI 1640 medium containing different resveratrol concentrations. A549 cells were transfected and grouped as follows: blank group, siRNA-negative control group, siRNA-COX-2 group and resveratrol + siRNA-COX-2 group. qRT-PCR and Western blot were conducted to detect COX-2 expression. MTT assay, soft agar clone assay and flow cytometry were performed to assess proliferation and cell cycle. Results: The relative expression of COX-2 mRNA was significantly increased in lung adenocarcinoma tissues (P<0.01) and it was closely related with clinical stages. Resveratrol at 60 µmol/L significantly inhibited A549 cells proliferation, S phase cells proportion and COX-2 expression (P<0.01). COX-2 expression in siRNA-COX-2 group was significantly lower than that in blank group and siRNA-negative control group (P<0.01). OD570 values, colony formation rate and S phase cells proportion of resveratrol + siRNA-COX-2 group were much lower than those of other groups (P<0.01). Conclusion: Resveratrol inhibits A549 cells proliferation by inhibiting COX-2 expression. Keywords: resveratrol, COX-2 expression, A549 cell, proliferation

Keywords

• Resveratrol
• COX-2 expression
• A549 cell
• proliferation