In Vivo Biocompatibility of Dental Bonding Agents containing Colloidal Dispersion of Titanium Dioxide

Abstract

Purpose: To investigate the direct and indirect cytotoxic effects of two universal dental bonding agents incorporated with titanium oxide colloidal dispersion on a human gingival fibroblast cell. Materials and Methods: Two commercial dental bonding agents’ systems, i.e., Ambar universal (FGM, Brasil) and G-Premio Bond Universal (GC, America) were incorporated with 4% by mass of colloidal dispersion containing titanium oxide (TiO2) nanoparticles. Human gingival fibroblast cells were used for cytocompatibility analysis. Two cytotoxic assays were used to investigate the cytotoxic activity of four bonding agent groups on the fibroblast-like cells as follows; GA: Ambar Universal (control), GB: Ambar Universal (4% TiO2 incorporated), GC: G-Premio Bond universal (control), and GD: G-Premio Bond (4% TiO2 incorporated). Forty bonding agent samples (5 x 1 mm discs) were prepared from the bonding agent groups and used for 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay and 32 discs were used for the High-Content Screening (HCS) assay. Results: The results from the cytotoxic assays showed a high degree of cytocompatibility for all tested bonding agents. However, the incorporated bonding agent Groups (GB and GD) showed significantly less cytotoxic effects than their controls. Also, groups GC and GD showed significantly higher cytocompatibility than GA and GB. Conclusion: The incorporation of 4% by mass of colloidal dispersion of titanium oxide nanoparticles significantly enhanced the biocompatibility of the tested universal bonding agents in comparison to their control groups.

Keywords

• Universal Bonding Agents
• Colloidal Dispersion
• Titanium Oxide
• Cytocompatibility