Cytotoxicity and Antimicrobial Activity of BioAkt^TM and Phytic Acid: A Laboratory-Based Study

Abstract

(1) Background: To improve endodontic treatments, it is necessary to find good irrigant solutions that balance potential toxic effects with optimal antimicrobial capacity. The aim of this laboratory study was to assess the cytotoxic and antimicrobial capacity of phytic acid (IP6) and BioAktTM in a laboratory setting to determine their suitability prior to endodontic evaluation. (2) Methods: The antimicrobial and cytotoxic effects of IP6 and BioAktTM were compared with those of 1.5% NaOCl. Cytotoxicity was evaluated via crystal violet assay and CellTiter-Glo® 2.0 assay, while antimicrobial capacity was tested via disk diffusion and the effect of a 1-h treatment on Enterococcus faecalis. (3) Results: A crystal violet test and CellTiter-Glo® 2.0 assay showed statistically significant differences in cell viability and cell activity after 4 and 24 h for all tested solutions (p TM was similar to that of 1.5% NaOCl, while phytic acid showed the lowest antimicrobial activity compared to BioAktTM (p p TM showed an optimal balance between antimicrobial activity and cytotoxicity.

Keywords

• BioAkt^TM
• phytic acid
• cytotoxicity
• antimicrobial activity
• laboratory study