Live Imaging of Axonal Transport in the Motor Neurons of Drosophila Larvae
Tsuyoshi Inoshita,
Nobutaka Hattori,
Yuzuru Imai
Affiliations
Tsuyoshi Inoshita
Department of Research for Parkinson’s Disease, Juntendo University Graduate School of Medicine, Tokyo, Japan
Nobutaka Hattori
Department of Research for Parkinson’s Disease, Juntendo University Graduate School of Medicine, Tokyo, JapanDepartment of Neurology, Juntendo University Graduate School of Medicine, Tokyo, Japan
Yuzuru Imai
Department of Research for Parkinson’s Disease, Juntendo University Graduate School of Medicine, Tokyo, Japan
Axonal transport, which is composed of microtubules, motor proteins and a variety of types of cargo, is a prominent feature of neurons. Monitoring these molecular dynamics is important to understand the biological processes of neurons as well as neurodegenerative disorders that are associated with axonal dysfunction. Here, we describe a protocol for monitoring the axonal transport of motor neurons in Drosophila larvae using inverted fluorescence microscopy.
