Structure of the G protein chaperone and guanine nucleotide exchange factor Ric-8A bound to Gαi1

Levi J. McClelland; Kaiming Zhang; Tung-Chung Mou; Jake Johnston; Cindee Yates-Hansen; Shanshan Li; Celestine J. Thomas; Tzanko I. Doukov; Sarah Triest; Alexandre Wohlkonig; Gregory G. Tall; Jan Steyaert; Wah Chiu; Stephen R. Sprang

doi:10.1038/s41467-020-14943-4

Nature Communications (Feb 2020)

Structure of the G protein chaperone and guanine nucleotide exchange factor Ric-8A bound to Gαi1

Levi J. McClelland,
Kaiming Zhang,
Tung-Chung Mou,
Jake Johnston,
Cindee Yates-Hansen,
Shanshan Li,
Celestine J. Thomas,
Tzanko I. Doukov,
Sarah Triest,
Alexandre Wohlkonig,
Gregory G. Tall,
Jan Steyaert,
Wah Chiu,
Stephen R. Sprang

Affiliations

Levi J. McClelland: Center for Biomolecular Structure and Dynamics, University of Montana
Kaiming Zhang: Department of Bioengineering and James H. Clark Center, Stanford University
Tung-Chung Mou: Center for Biomolecular Structure and Dynamics, University of Montana
Jake Johnston: Center for Biomolecular Structure and Dynamics, University of Montana
Cindee Yates-Hansen: Center for Biomolecular Structure and Dynamics, University of Montana
Shanshan Li: Department of Bioengineering and James H. Clark Center, Stanford University
Celestine J. Thomas: Center for Biomolecular Structure and Dynamics, University of Montana
Tzanko I. Doukov: Macromolecular Crystallography Group, Stanford Synchrotron Radiation Light Source, SLAC National Accelerator Laboratory, Stanford University
Sarah Triest: Structural Biology Brussels, Vrije Universiteit Brussel (VUB)
Alexandre Wohlkonig: Structural Biology Brussels, Vrije Universiteit Brussel (VUB)
Gregory G. Tall: Department of Pharmacology, University of Michigan Medical School
Jan Steyaert: Structural Biology Brussels, Vrije Universiteit Brussel (VUB)
Wah Chiu: Department of Bioengineering and James H. Clark Center, Stanford University
Stephen R. Sprang: Center for Biomolecular Structure and Dynamics, University of Montana

DOI: https://doi.org/10.1038/s41467-020-14943-4
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

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Ric-8A is guanine nucleotide exchange factor (GEF) that also acts as a folding chaperone for its Gα subunit. Here, the authors present the structure of Ric-8A bound to nucleotide-free Gαi1, revealing the mechanism by which Ric-8A exerts both GEF and chaperone activity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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