A distinct mechanism of C-type inactivation in the Kv-like KcsA mutant E71V

Ahmed Rohaim; Bram J. A. Vermeulen; Jing Li; Felix Kümmerer; Federico Napoli; Lydia Blachowicz; João Medeiros-Silva; Benoît Roux; Markus Weingarth

doi:10.1038/s41467-022-28866-9

Nature Communications (Mar 2022)

A distinct mechanism of C-type inactivation in the Kv-like KcsA mutant E71V

Ahmed Rohaim,
Bram J. A. Vermeulen,
Jing Li,
Felix Kümmerer,
Federico Napoli,
Lydia Blachowicz,
João Medeiros-Silva,
Benoît Roux,
Markus Weingarth

Affiliations

Ahmed Rohaim: Department of Biochemistry and Molecular Biology, The University of Chicago
Bram J. A. Vermeulen: NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University
Jing Li: Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi
Felix Kümmerer: NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University
Federico Napoli: NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University
Lydia Blachowicz: Department of Biochemistry and Molecular Biology, The University of Chicago
João Medeiros-Silva: NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University
Benoît Roux: Department of Biochemistry and Molecular Biology, The University of Chicago
Markus Weingarth: NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University

DOI: https://doi.org/10.1038/s41467-022-28866-9
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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Constriction of the selectivity filter is assumed to be a hallmark of C-type inactivation in K+ channels. Using different high-resolution methods, this study shows a distinct C-type inactivation mechanism in a KcsA mutant that emulates Kv-channels.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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