Protocol for immunophenotyping out-of-hospital cardiac arrest patients

Abstract

Summary: Immunophenotyping of out-of-hospital cardiac arrest (OHCA) patients is of increasing interest but has challenges. Here, we describe steps for the design of the clinical cohort, planning patient enrollment and sample collection, and ethical review of the study protocol. We detail procedures for blood sample collection and cryopreservation of peripheral blood mononuclear cells (PBMCs). We detail steps to modulate immune checkpoints in OHCA PBMC ex vivo. This protocol also has relevance for immunophenotyping other types of critical illness.For complete details on the use and execution of this protocol, please refer to Tamura et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Clinical Protocol
• Flow Cytometry/Mass Cytometry
• Immunology
• RNA-seq
• Single Cell