Bio-Protocol (Aug 2020)

In-vitro GLP-1 Release Assay Using STC-1 Cells

  • Liu Qi,
  • Tan Shuai,
  • Jia Da,
  • Burstein Ezra,
  • Sifuentes-Dominguez Luis

DOI
https://doi.org/10.21769/BioProtoc.3717
Journal volume & issue
Vol. 10, no. 16

Abstract

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Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.