Single Cell Sequencing of the Pineal Gland: The Next Chapter

Steven L. Coon; Cong Fu; Cong Fu; Steven W. Hartley; Lynne Holtzclaw; Joseph C. Mays; Michael C. Kelly; Matthew W. Kelley; James C. Mullikin; Martin F. Rath; Luis E. Savastano; David C. Klein

doi:10.3389/fendo.2019.00590

Frontiers in Endocrinology (Sep 2019)

Single Cell Sequencing of the Pineal Gland: The Next Chapter

Steven L. Coon,
Cong Fu,
Cong Fu,
Steven W. Hartley,
Lynne Holtzclaw,
Joseph C. Mays,
Michael C. Kelly,
Matthew W. Kelley,
James C. Mullikin,
Martin F. Rath,
Luis E. Savastano,
David C. Klein

Affiliations

Steven L. Coon: Molecular Genomics Core, Office of the Scientific Director, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, United States
Cong Fu: Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education, The First Hospital of Jilin University, Changchun, China
Cong Fu: National-Local Joint Engineering Laboratory of Animal Models for Human Diseases, Changchun, China
Steven W. Hartley: Comparative Genomics Analysis Unit, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, United States
Lynne Holtzclaw: Microscopy and Imaging Core, Office of the Scientific Director, Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, United States
Joseph C. Mays: Institute on Systems Genetics, New York University School of Medicine, New York, NY, United States
Michael C. Kelly: Single Cell Analysis Facility, Frederick National Lab for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States
Matthew W. Kelley: Section on Developmental Neuroscience, Laboratory of Cochlear Development, Division of Intramural Research, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD, United States
James C. Mullikin: National Institutes of Health Intramural Sequencing Center, National Human Genome Research Institute, National Institutes of Health, Rockville, MD, United States
Martin F. Rath: 0Department of Neuroscience, Panum Institute, University of Copenhagen, Copenhagen, Denmark
Luis E. Savastano: 1Department of Neurosurgery, University of Michigan, Ann Arbor, MI, United States
David C. Klein: 2Office of the Scientific Director, Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, United States

DOI: https://doi.org/10.3389/fendo.2019.00590
Journal volume & issue: Vol. 10

Abstract

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The analysis of pineal cell biology has undergone remarkable development as techniques have become available which allow for sequencing of entire transcriptomes and, most recently, the sequencing of the transcriptome of individual cells. Identification of at least nine distinct cell types in the rat pineal gland has been made possible, allowing identification of the precise cells of origin and expression of transcripts for the first time. Here the history and current state of knowledge generated by these transcriptomic efforts is reviewed, with emphasis on the insights suggested by the findings.

Published in Frontiers in Endocrinology

ISSN: 1664-2392 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the endocrine glands. Clinical endocrinology
Website: https://www.frontiersin.org/journals/endocrinology

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