Nature Communications (Nov 2021)
Identification of proximal SUMO-dependent interactors using SUMO-ID
- Orhi Barroso-Gomila,
- Fredrik Trulsson,
- Veronica Muratore,
- Iñigo Canosa,
- Laura Merino-Cacho,
- Ana Rosa Cortazar,
- Coralia Pérez,
- Mikel Azkargorta,
- Ibon Iloro,
- Arkaitz Carracedo,
- Ana M. Aransay,
- Felix Elortza,
- Ugo Mayor,
- Alfred C. O. Vertegaal,
- Rosa Barrio,
- James D. Sutherland
Affiliations
- Orhi Barroso-Gomila
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- Fredrik Trulsson
- Cell and Chemical Biology, Leiden University Medical Center (LUMC)
- Veronica Muratore
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- Iñigo Canosa
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- Laura Merino-Cacho
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- Ana Rosa Cortazar
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- Coralia Pérez
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- Mikel Azkargorta
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- Ibon Iloro
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- Arkaitz Carracedo
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- Ana M. Aransay
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- Felix Elortza
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- Ugo Mayor
- Ikerbasque, Basque Foundation for Science
- Alfred C. O. Vertegaal
- Cell and Chemical Biology, Leiden University Medical Center (LUMC)
- Rosa Barrio
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- James D. Sutherland
- Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park
- DOI
- https://doi.org/10.1038/s41467-021-26807-6
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 19
Abstract
Several proteomic approaches allow the analysis of covalent protein SUMOylation, but it remains challenging to systematically study the consequences of a substrate being modified. Here, the authors combine proximity biotinylation and protein-fragment complementation to identify SUMO-dependent protein interactors.
