A modified behavioral test protocol for simultaneously evaluating the incentive value of alcohol drinking and social interaction in mice
Haodong Su,
Ye He,
Yuying Li,
Kangguang Lin,
Guiyun Xu,
Tifei Yuan,
Zhe Shi,
Chunlu Li
Affiliations
Haodong Su
School of Humanities and Social Sciences, Binzhou Medical University, Yantai, China
Ye He
The Affiliated Brain Hospital of Guangzhou Medical University, (Guangzhou Hu-iai Hospital), Guangzhou Medical University, Guangzhou, China
Yuying Li
The Affiliated Brain Hospital of Guangzhou Medical University, (Guangzhou Hu-iai Hospital), Guangzhou Medical University, Guangzhou, China
Kangguang Lin
The Affiliated Brain Hospital of Guangzhou Medical University, (Guangzhou Hu-iai Hospital), Guangzhou Medical University, Guangzhou, China
Guiyun Xu
The Affiliated Brain Hospital of Guangzhou Medical University, (Guangzhou Hu-iai Hospital), Guangzhou Medical University, Guangzhou, China
Tifei Yuan
Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai JiaoTong University School of Medicine, Shanghai, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China; Corresponding authors.
Zhe Shi
Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Corresponding authors.
Chunlu Li
School of Humanities and Social Sciences, Binzhou Medical University, Yantai, China; Department of Psychology, School of Medical Humanitarians, Guizhou Medical University, Guiyang, China; Corresponding author at: Department of Psychology, School of Medical Humanitarians, Guizhou Medical University, Guiyang, China.
Individual sociability and alcohol drinking are interacted to escalate alcohol use. An impairment in perceiving and discriminating the difference in incentive values between social interaction and drinking behavior indicates a shift from moderate alcohol consumption to misuse. However, few studies have evaluated the incentive value of these two behaviors in the same scenario. Thus, we modified a behavioral test protocol to evaluate rodents’ ability to perceive and discriminate the differences in incentive value between alcohol drinking and interaction with their social partners. The present protocol is simple and practicable. Only 2–3 days are required to complete the whole process. Compared with existing methods, our protocol is simple and practicable. Our findings suggested that subtle changes in the incentive value of distinct behaviors can be accurately and reliably assessed using the present protocol in mice with low or high levels of alcohol preference. • We described a modified behavioral test protocol to simultaneously evaluate the incentive value of alcohol drinking and social interaction. • The subtle changes in the incentive value of mice with different levels of alcohol preference can be accurately and reliably assessed in the present protocol. • Using our modified protocol, the differences of incentive value between distinct behaviors can be accurately and reliably assessed in mice with different risks to develop into AUD.
