Egg white hydrolysate peptides act as antimicrobial and anti-inflammatory agents for acne
Anuwat Wanthong,
Chanapat Boonmark,
Nichakamol Vaisopha,
Sittiruk Roytrakul,
Anupong Tankrathok,
Lapatrada Taemaitree,
Sakda Daduang,
Sophon Boonlue,
Watcharee Khunkitti,
Sompong Klaynongsruang,
Nisachon Jangpromma
Affiliations
Anuwat Wanthong
Department of Biology, Faculty of Science, Mahasarakham University, Mahasarakham, 44150, Thailand; Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand
Chanapat Boonmark
Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand
Functional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, 12120, Thailand
Anupong Tankrathok
Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Biotechnology, Faculty of Agricultural Technology, Kalasin University, Kalasin, 46000, Thailand
Lapatrada Taemaitree
Department of Integrated Science, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand
Sakda Daduang
Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand
Sophon Boonlue
Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand
Watcharee Khunkitti
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Science, Khon Kaen University, Khon Kaen, 40002, Thailand
Sompong Klaynongsruang
Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand
Nisachon Jangpromma
Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand; Corresponding author. Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
A simple method to generate antibacterial peptides by alkaline hydrolysis of hen egg whites is reported. The method reproducibly generates short peptides with molecular weight of less than 14.4 kDa that exhibit low to no cytotoxicity on RAW 264.7 macrophage cells, but do inhibit the bacterial growth of Cutibacterium acnes (C. acnes), Staphylococcus aureus (S. aureus) and antibiotic-resistant S. aureus (MRSA), while also reducing nitric oxide production from heat-killed C. acnes-treated RAW 264.7 cells. Peptidomics revealed at least thirty peptides within the complex mixture, of which eight were evaluated individually. Three peptides (PK8, EE9 and RP8) were potent anti-inflammation and antibacterial agents, but notably the complex egg white hydrolysate (EWH) was more effective than the individual peptides. Electron microscopy suggests the antibacterial mechanism of both the hydrolysate and the selected peptides is through disruption of the cell membrane of C. acnes. These findings suggest that EWH and EWH-derived peptides are promising candidates for infection and inflammation treatment, particularly in managing acne and combating antibiotic-resistant bacteria like MRSA.
