The Influence of Ozone on Net Ecosystem Production of a Ryegrass–Clover Mixture under Field Conditions

Abstract

In order to understand the effect of phytotoxic tropospheric ozone (O3) on terrestrial vegetation, we quantified the impact of current O3 concentration ([O3]) on net ecosystem production (NEP) when compared to the conditions of the pre-industrial era. We compared and tested linear mixed-effects models based on [O3] and stomatal O3 flux (Fsto). The managed ryegrass–clover (Lolium perenne and Trifolium pratense) mixture was grown on arable land in the Czech Republic, Central Europe. Values of [O3] and Fsto were measured and calculated based on resistance analogy, respectively, while NEP was calculated from eddy covariance CO2 fluxes. We found the Fsto-based model more precise when compared to measured NEP. High Fsto was found even at low [O3], while broad summer maximum of [O3] was not necessarily followed by significant NEP decline, due to low soil water content leading to a low stomatal conductivity and Fsto. Comparing to low pre-industrial O3 conditions, current levels of O3 resulted in the reduction of cumulative NEP over the entire growing season, up to 29.7 and 13.5% when the [O3]-based and Fsto-based model was applied, respectively. During the growing season, an O3-induced reduction of NEP ranged between 13.1% in May and 26.2% in July when compared to pre-industrial Fsto levels. Looking to the future, high [O3] and Fsto may lead to the reduction of current NEP by approximately 13.3% on average during the growing season, but may increase by up to 61–86.6% in autumn, indicating further O3-induced acceleration of the senescence. These findings indicate the importance of Fsto and its inclusion into the models estimating O3 effects on terrestrial vegetation. The interaction between environmental factors and stomatal conductance is therefore discussed in detail.

Keywords

• eddy covariance
• linear mixed-effects model
• pre-industrial O₃ conditions
• seasonal carbon uptake
• stomatal ozone flux