Stem Cell Research (Mar 2016)

Derivation of human embryonic stem cell line Genea023

  • Biljana Dumevska,
  • Alexis Bosman,
  • Robert McKernan,
  • Divya Goel,
  • Uli Schmidt,
  • Teija Peura

DOI
https://doi.org/10.1016/j.scr.2016.02.012
Journal volume & issue
Vol. 16, no. 2
pp. 456 – 459

Abstract

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The Genea023 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XY karyotype and male allele pattern through CGH and STR analysis. Pluripotency of Genea023 was demonstrated with 85% of cells expressed Nanog, 98% Oct4, 55% Tra1-60 and 98% SSEA4, gave a Pluritest Pluripotency score of 42.76, Novelty of 1.23, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.