Improvement of combining abilities of sugar beet population C-8173 using in vitro methods

Abstract

The ultra sugary cultivar Crvenka mz, released in 1997, is a triploid whose parental components are the monogerm cms line A-0401 and the multigerm tetraploid population C-8173. The objective of our study was to select from the male parental component superior genotypes that are good combiners and thereby develop anew, improved population. Based on the phenotype. 400 roots of the C-8173 pollinator were chosen in the field. Later that year. 106 of them were selected on the basis of root mass and refraction. In the spring of the following year, each of these genotypes was transplanted onto a special plot with six roots of the female parental component A-0401. Hemp was used as the isolator. In vitro micro propagation from lateral head buds was used in the selected genotypes to carry out the recurrent selection process. In 1999. a small-plot trial with successful hybrids was established in which the hybrid cultivar Crvenka mz was used as the standard. Based on the hybrids' production characteristics, 20 superior genotypes were chosen, which were then maintained in vitro and propagated using methods of plant regeneration through the formation of axillary buds. Their hybrids have proven to be significantly better than the standard cultivar and will be used in further research to develop an improved pollinator population. The selected hybrids outperformed the standard in the following areas: root yield (by 12.2%), sugar content (by 2.2%. in relative terms), raw sugar yield (by 14.4%), and white sugar yield (by 15.0%). .