Effect of methotrexate on neurobehavior and cholinesterase in chicks

Abstract

This reading aimed to determine the therapeutic index of methotrexate (MTX) with a note on the consequence of methotrexate in chicks on neurobehavioral in addition to calculating the activity for cholinesterase in plasma and brain in chicks. Two groups with 10 chicks each were utilized once. The untreated and methotrexate groups were given saline and MTX at dose 40 mg/kg for 5 days, respectively. The methotrexate group obtained considerable amounts of methotrexate 9th day of the start of the experiment for five days. All chicks were exposed to open-field activity and tonic immobility tests. Brain tissue with blood samples were collected to evaluate cholinesterase levels in chicks. ED50 and LD50 for methotrexate in the chicks were 14.36mg/ kg and102.63 mg/ kg, correspondingly. In addition, Therapeutic index of methotrexate was 7.15. The methotrexate group explained an important enhance in latency to move and tonic immobility evaluated with an untreated group, there is a significant decline in lines crossed, and escape jumps compared with untreated group, and the cholinesterase activity showed a significantly reduced in plasma and brain samples treated with methotrexate and inhibition expressed significantly increased evaluated through the control group. Our findings conclude that methotrexate has a depressant action on the nervous structure and an inhibitory action on the cholinesterase level in the chick’s model.

Keywords

• therapeutic index
• cholinesterase
• median effective dose
• methotrexate
• chicks