Frontiers in Physics (Sep 2024)

Structured illumination lensless digital holographic microscopy (SI-LDHM)

  • Juanjuan Zheng,
  • Juanjuan Zheng,
  • Juanjuan Zheng,
  • Xuhong Guo,
  • Xuhong Guo,
  • Xuhong Guo,
  • Ying Ma,
  • Ying Ma,
  • Ying Ma,
  • Kai Wen,
  • Kai Wen,
  • Kai Wen,
  • Sha An,
  • Sha An,
  • Sha An,
  • Xiaofang Wang,
  • Xiaofang Wang,
  • Xiaofang Wang,
  • Peng Gao,
  • Peng Gao,
  • Peng Gao,
  • Jiaming Qian,
  • Chao Zuo,
  • Peng Gao

DOI
https://doi.org/10.3389/fphy.2024.1485687
Journal volume & issue
Vol. 12

Abstract

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In this work, we propose a structured-illumination lensless digital holographic microscopy (SI-LDHM). SI-LDHM illuminates a sample with 24 structured illuminations (8 orientations × 3 phase shifts) and records the defocused interferogram formed by two copies of object waves along the ±1st diffraction orders of each SI. The reconstructed object waves under different illumination orientations are respectively propagated to the sample plane along the +1st diffraction order and then averaged, thus yielding a clean image without the artifact of twin images. Experimental results demonstrated that thanks to the multi-oriented SI strategy, the twin image in SI-LDHM is sevenfold reduced compared to conventional DHM, while the spatial resolution is 1.15 times higher.

Keywords