Fine-tuning sugar content in strawberry

Sinian Xing; Kunling Chen; Haocheng Zhu; Rui Zhang; Huawei Zhang; Bingbing Li; Caixia Gao

doi:10.1186/s13059-020-02146-5

Genome Biology (Sep 2020)

Fine-tuning sugar content in strawberry

Sinian Xing,
Kunling Chen,
Haocheng Zhu,
Rui Zhang,
Huawei Zhang,
Bingbing Li,
Caixia Gao

Affiliations

Sinian Xing: State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Kunling Chen: State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Haocheng Zhu: State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Rui Zhang: State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Huawei Zhang: State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences
Bingbing Li: College of Horticulture, China Agricultural University
Caixia Gao: State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences

DOI: https://doi.org/10.1186/s13059-020-02146-5
Journal volume & issue: Vol. 21, no. 1
pp. 1 – 14

Abstract

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Abstract Fine-tuning quantitative traits for continuous subtle phenotypes is highly advantageous. We engineer the highly conserved upstream open reading frame (uORF) of FvebZIPs1.1 in strawberry (Fragaria vesca), using base editor A3A-PBE. Seven novel alleles are generated. Sugar content of the homozygous T1 mutant lines is 33.9–83.6% higher than that of the wild-type. We also recover a series of transgene-free mutants with 35 novel genotypes containing a continuum of sugar content. All the novel genotypes could be immediately fixed in subsequent generations by asexual reproduction. Genome editing coupled with asexual reproduction offers tremendous opportunities for quantitative trait improvement.

Published in Genome Biology

ISSN: 1474-760X (Online)
Publisher: BMC
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General): Genetics
Website: https://genomebiology.biomedcentral.com/

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