Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties

Elmira Jalilian; Hamed Massoumi; Bianca Bigit; Sohil Amin; Eitan A. Katz; Victor H. Guaiquil; Khandaker N. Anwar; Peiman Hematti; Mark I. Rosenblatt; Ali R. Djalilian

doi:10.1186/s13287-022-03128-z

Stem Cell Research & Therapy (Aug 2022)

Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties

Elmira Jalilian,
Hamed Massoumi,
Bianca Bigit,
Sohil Amin,
Eitan A. Katz,
Victor H. Guaiquil,
Khandaker N. Anwar,
Peiman Hematti,
Mark I. Rosenblatt,
Ali R. Djalilian

Affiliations

Elmira Jalilian: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago
Hamed Massoumi: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago
Bianca Bigit: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago
Sohil Amin: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago
Eitan A. Katz: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago
Victor H. Guaiquil: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago
Khandaker N. Anwar: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago
Peiman Hematti: Department of Medicine, Hematology/Oncology Division, University of Wisconsin-Madison, School of Medicine and Public Health
Mark I. Rosenblatt: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago
Ali R. Djalilian: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago

DOI: https://doi.org/10.1186/s13287-022-03128-z
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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Highlights Extracellular vesicles (EV) derived from 2D and 3D bone marrow mesenchymal stromal cells (BM-MSCs) demonstrate different characterization phenotype. Heterogeneity and concentration of BM-MSCs-derived extracellular vesicles are highly impacted by the culture conditions. 3D-derived EVs can significantly enhance trigeminal nerves elongation and complexity compared to 2D-derived EVs. 3D culture conditions are highly efficient environment for upscale production of MSC-derived EVs with enhanced neuro-regeneration properties for corneal nerve regeneration.

Published in Stem Cell Research & Therapy

ISSN: 1757-6512 (Online)
Publisher: BMC
Country of publisher: United Kingdom
LCC subjects: Medicine: Medicine (General); Science: Chemistry: Organic chemistry: Biochemistry
Website: https://stemcellres.biomedcentral.com

About the journal

Abstract

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