Nature Communications (Feb 2022)

Neutralizing antibodies induced in immunized macaques recognize the CD4-binding site on an occluded-open HIV-1 envelope trimer

  • Zhi Yang,
  • Kim-Marie A. Dam,
  • Michael D. Bridges,
  • Magnus A. G. Hoffmann,
  • Andrew T. DeLaitsch,
  • Harry B. Gristick,
  • Amelia Escolano,
  • Rajeev Gautam,
  • Malcolm A. Martin,
  • Michel C. Nussenzweig,
  • Wayne L. Hubbell,
  • Pamela J. Bjorkman

DOI
https://doi.org/10.1038/s41467-022-28424-3
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 14

Abstract

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Neutralizing antibodies (bNAbs) against HIV-1 are exclusively directed against the viral envelope protein (Env) and mainly target Env in a closed, prefusion state. Here, Yang et al. structurally characterize two heterologously-neutralizing CD4-binding site (CD4bs) antibodies isolated from sequentially immunized macaques, and show that these antibodies recognize the CD4bs on Env trimers in an „occluded-open‟ conformation between closed, as targeted by bNAbs, and fully-open, as recognized by CD4.