A fabricated hydrogel of hyaluronic acid/curcumin shows super-activity to heal the bacterial infected wound
Maryam Khaleghi,
Fakhri Haghi,
Mina Gholami,
Hamdam Hourfar,
Farshad Shahi,
Ali Mir Mousavi Zekoloujeh,
Farhang Aliakbari,
Ebrahim Ahmadi,
Dina Morshedi
Affiliations
Maryam Khaleghi
Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology
Fakhri Haghi
Department of Microbiology, School of Medicine, Zanjan University of Medical Sciences
Mina Gholami
Department of Microbiology, School of Medicine, Zanjan University of Medical Sciences
Hamdam Hourfar
Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology
Farshad Shahi
Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology
Ali Mir Mousavi Zekoloujeh
Department of Biology, University of Zanjan
Farhang Aliakbari
Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology
Ebrahim Ahmadi
Department of Chemistry, University of Zanjan
Dina Morshedi
Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology
Abstract High risk of acute morbidities and even mortality from expanding the antibiotics resistant infectious wounds force indefinite efforts for development of high performance wound-healing materials. Herein, we design a procedure to fabricate a hyaluronic acid (HA)-based hydrogel to conjugate curcumin (Gel-H.P.Cur). The highlight of this work is to provide a favorite condition for capturing curcumin while protecting its structure and intensifying its activities because of the synchronization with HA. Accordingly, HA as a major component of dermis with a critical role in establishing skin health, could fortify the wound healing property as well as antibacterial activity of the hydrogel. Gel-H.P.Cur showed antibacterial properties against Pseudomonas aeruginosa (P. aeruginosa), which were examined by bactericidal efficiency, disk diffusion, anti-biofilm, and pyocyanin production assays. The effects of Gel-H.P.Cur on the inhibition of quorum sensing (QS) regulatory genes that contribute to expanding bacteria in the injured place was also significant. In addition, Gel-H.P.Cur showed high potential to heal the cutaneous wounds on the mouse excisional wound model with repairing histopathological damages rapidly and without scar. Taken together, the results strongly support Gel-H.P.Cur as a multipotent biomaterial for medical applications regarding the treatment of chronic, infected, and dehiscent wounds.
