Nature Communications (Feb 2021)

Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria

  • Zhe Sun,
  • Alexander V. Yakhnin,
  • Peter C. FitzGerald,
  • Carl E. Mclntosh,
  • Mikhail Kashlev

DOI
https://doi.org/10.1038/s41467-021-21150-2
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 14

Abstract

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Transcription by bacterial RNA polymerase is interrupted by pausing events that play diverse regulatory roles. Here, the authors find that a large number of E. coli sigma70-dependent pauses, clustered at a 10−20-bp distance from promoters, are regulated by Gre cleavage factors constituting a mechanism for rapid response to changing environmental cues.