Overexpression of the peroxidase gene ZmPRX1 increases maize seedling drought tolerance by promoting root development and lignification

Xiuzhen Zhai; Xiaocui Yan; Tinashe Zenda; Nan Wang; Anyi Dong; Qian Yang; Yuan Zhong; Yue Xing; Huijun Duan

Crop Journal (Jun 2024)

Overexpression of the peroxidase gene ZmPRX1 increases maize seedling drought tolerance by promoting root development and lignification

Xiuzhen Zhai,
Xiaocui Yan,
Tinashe Zenda,
Nan Wang,
Anyi Dong,
Qian Yang,
Yuan Zhong,
Yue Xing,
Huijun Duan

Affiliations

Xiuzhen Zhai: State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Crop Germplasm Resources in North China, Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, Hebei, China
Xiaocui Yan: State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Crop Germplasm Resources in North China, Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, Hebei, China
Tinashe Zenda: State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Crop Germplasm Resources in North China, Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, Hebei, China
Nan Wang: State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Crop Germplasm Resources in North China, Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, Hebei, China
Anyi Dong: State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Crop Germplasm Resources in North China, Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, Hebei, China
Qian Yang: State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Crop Germplasm Resources in North China, Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, Hebei, China
Yuan Zhong: State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Crop Germplasm Resources in North China, Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, Hebei, China
Yue Xing: State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Crop Germplasm Resources in North China, Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, Hebei, China
Huijun Duan: Corresponding author.; State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Crop Germplasm Resources in North China, Ministry of Education, College of Agronomy, Hebei Agricultural University, Baoding 071001, Hebei, China

Journal volume & issue: Vol. 12, no. 3
pp. 753 – 765

Abstract

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Drought is a main abiotic stress factor hindering plant growth, development, and crop productivity. Therefore, it is crucial to understand the mechanisms by which plants cope with drought stress. Here, the function of the maize peroxidase gene ZmPRX1 in drought stress tolerance was investigated by measurement of its expression in response to drought treatment both in a ZmPRX1 overexpression line and a mutant line. The higher root lignin accumulation and seedling survival rate of the overexpression line than that of the wild type or mutant support a role for ZmPRX1 in maize drought tolerance by regulating root development and lignification. Additionally, yeast one-hybrid, Dule luciferase and ChIP-qPCR assays showed that ZmPRX1 is negatively regulated by a nuclear-localized ZmWRKY86 transcription factor. The gene could potentially be used for breeding of drought-tolerant cultivars.

Published in Crop Journal

ISSN: 2095-5421 (Print); 2214-5141 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.keaipublishing.com/en/journals/the-crop-journal/

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