Microsatellite Markers for <i>Leucobryum boninense</i> (Leucobryaceae), Endemic to the Bonin Islands, Japan

Abstract

Premise of the study: Microsatellite primers were developed for Leucobryum boninense, endemic to the Bonin Islands, Japan, to investigate its level of genetic diversity and population genetic structure. Methods and Results: Using next-generation sequencing, 21 primer sets were developed, among which nine loci were polymorphic in the populations of the Bonin Islands. Among these polymorphic loci, the number of alleles per locus ranged from two to 10 (mean = 3.444) and the expected heterozygosity ranged from 0.066 to 0.801 (mean = 0.338). Conclusions: These results indicate the utility of the nine microsatellite markers that we developed for population genetic studies of L. boninense.

Keywords

• Bonin (Ogasawara) Islands
• Leucobryaceae
• Leucobryum boninense
• microsatellite
• Musci
• next-generation sequencing