Absorption Patterns of Fucoidan Oligosaccharides from <i>Kjellmaniella crassifolia</i> in the Caco-2 Monolayer Cell Model and Their Pharmacokinetics in Mice

Abstract

Oligosaccharides possess characteristics such as low molecular weight, good solubility, and high bioavailability, which make them better absorbed than fucoidan. This study hypothesizes that fucoidan oligosaccharides can be absorbed by intestinal epithelial cells and quickly enter the bloodstream, with a rapid absorption rate. In this study, fucoidan oligosaccharides were obtained through acid degradation and Bio Gel column separation. By analyzing the chemical composition and molecular weight, oligosaccharides with smaller molecular weights and simpler monosaccharide compositions were selected for further research. A cell model and pharmacokinetic studies in mice were established to analyze the absorption patterns of the oligosaccharides. The results showed that after acid degradation and column separation, high-molecular-weight oligosaccharides SPF1 with a molecular weight range of 1.63 × 104 to 2.14 × 105 Da and the low-molecular-weight oligosaccharides SPF2 with a molecular weight range of 244.22 to 1545.36 Da were obtained. In cell transport and uptake experiments, the transport of SPF1 and SPF2 was positively correlated with time and negatively correlated with concentration. The transport rates of SPF1 and SPF2 ranged from 20% to 70%, with Papp values greater than 1 × 10−5 cm/s. In the pharmacokinetics study, the blood concentration of the oligosaccharides in mice was simulated and analyzed using DAS 2.0, which indicated that the fucoidan oligosaccharides exhibited good absorption characteristics in vivo and in vitro. Therefore, fucoidan oligosaccharides with smaller molecular weights are more easily absorbed, which provides a theoretical basis for the application of fucoidan oligosaccharides.

Keywords

• fucoidan oligosaccharides
• structural analysis
• column separation
• bidirectional transmembrane transport