Molecular Breeding of Water-Saving and Drought-Resistant Rice for Blast and Bacterial Blight Resistance
Anning Zhang,
Yi Liu,
Feiming Wang,
Deyan Kong,
Junguo Bi,
Fenyun Zhang,
Xingxing Luo,
Jiahong Wang,
Guolan Liu,
Lijun Luo,
Xinqiao Yu
Affiliations
Anning Zhang
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai Agrobiological Gene Center, Shanghai 201106, China
Yi Liu
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai Agrobiological Gene Center, Shanghai 201106, China
Feiming Wang
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai Agrobiological Gene Center, Shanghai 201106, China
Deyan Kong
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai Agrobiological Gene Center, Shanghai 201106, China
Junguo Bi
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai Agrobiological Gene Center, Shanghai 201106, China
Fenyun Zhang
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai Agrobiological Gene Center, Shanghai 201106, China
Xingxing Luo
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai Agrobiological Gene Center, Shanghai 201106, China
Jiahong Wang
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai Agrobiological Gene Center, Shanghai 201106, China
Guolan Liu
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai Agrobiological Gene Center, Shanghai 201106, China
Lijun Luo
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai Agrobiological Gene Center, Shanghai 201106, China
Xinqiao Yu
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai Agrobiological Gene Center, Shanghai 201106, China
Rice production is often affected by biotic and abiotic stressors. The breeding of resistant cultivars is a cost-cutting and environmentally friendly strategy to maintain a sustainable high production level. An elite water-saving and drought-resistant rice (WDR), Hanhui3, is susceptible to blast and bacterial blight (BB). This study was conducted to introgress three resistance genes (Pi2, xa5, and Xa23) for blast and BB into Hanhui3, using marker-assisted selection (MAS) for the foreground selection and a whole-genome single-nucleotide polymorphism (SNP) array for the background selection. As revealed by the whole-genome SNP array, the recurrent parent genome (RPG) recovery of the improved NIL was 94.2%. The resistance levels to blast and BB of the improved NIL and its derived hybrids were higher than that of the controls. In addition, the improved NIL and its derived hybrids retained the desired agronomic traits from Hanhui3, such as yield. The improved NIL could be useful to enhance resistance against biotic stressors and produce stable grain yields in Oryza sativa subspecies indica rice breeding programs.
