fMRI detects bilateral brain network activation following unilateral chemogenetic activation of direct striatal projection neurons
Yuki Nakamura,
Yukari Nakamura,
Assunta Pelosi,
Boucif Djemai,
Clément Debacker,
Denis Hervé,
Jean-Antoine Girault,
Tomokazu Tsurugizawa
Affiliations
Yuki Nakamura
Inserm UMR-S 1270, 75005, Paris, France; Sorbonne Université, Faculty of Sciences and Engineering, Paris, France; Institut du Fer à Moulin, 75005, Paris, France
Yukari Nakamura
Inserm UMR-S 1270, 75005, Paris, France; Sorbonne Université, Faculty of Sciences and Engineering, Paris, France; Institut du Fer à Moulin, 75005, Paris, France; Department of Pharmacology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan
Assunta Pelosi
Inserm UMR-S 1270, 75005, Paris, France; Sorbonne Université, Faculty of Sciences and Engineering, Paris, France; Institut du Fer à Moulin, 75005, Paris, France
Boucif Djemai
NeuroSpin, Commissariat à l’énergie Atomique et Aux énergies Alternatives, 91191, Gif-sur-Yvette, France
Clément Debacker
NeuroSpin, Commissariat à l’énergie Atomique et Aux énergies Alternatives, 91191, Gif-sur-Yvette, France
Denis Hervé
Inserm UMR-S 1270, 75005, Paris, France; Sorbonne Université, Faculty of Sciences and Engineering, Paris, France; Institut du Fer à Moulin, 75005, Paris, France
Jean-Antoine Girault
Inserm UMR-S 1270, 75005, Paris, France; Sorbonne Université, Faculty of Sciences and Engineering, Paris, France; Institut du Fer à Moulin, 75005, Paris, France; Corresponding author. Institut du Fer à Moulin, Inserm UMR-S1270, 17, rue du Fer à Moulin, 75005, Paris, France.
Tomokazu Tsurugizawa
NeuroSpin, Commissariat à l’énergie Atomique et Aux énergies Alternatives, 91191, Gif-sur-Yvette, France; Human Informatics and Interaction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8568, Japan; Corresponding author. NeuroSpin, Commissariat à l’Energie Atomique-Saclay Center, 91191, Gif-sur-Yvette, France.
Abnormal structural and functional connectivity in the striatum during neurological disorders has been reported using functional magnetic resonance imaging (fMRI), although the effects of cell-type specific neuronal stimulation on fMRI and related behavioral alterations are not well understood. In this study, we combined DREADD technology with fMRI (“chemo-fMRI”) to investigate alterations of spontaneous neuronal activity. These were induced by the unilateral activation of dopamine D1 receptor-expressing neurons (D1-neurons) in the mouse dorsal striatum (DS). After clozapine (CLZ) stimulation of the excitatory DREADD expressed in D1-neurons, the fractional amplitude of low frequency fluctuations (fALFF) increased bilaterally in the medial thalamus, nucleus accumbens and cortex. In addition, we found that the gamma-band of local field potentials was increased in the stimulated DS and cortex bilaterally. These results provide insights for better interpretation of cell type-specific activity changes in fMRI.
