To explain how the language system in the brain is temporally processed, the results of both brain and linguistics researches are required to be interpreted together. In this regard, phonologic functioning of language in the brain may be demonstrated more clearly by examining the auditory stimuli which are obtained via using auditory technique. This study focuses on acoustic preprocessing of auditory stimuli by using EEG technique in Bekâr (2016). This preprocessing is described as Stimulus Cross-Splicing method in this study. The auditory stimuli which was prepared in PRAAT 5.2 Sound Analysis Program with this method are interpreted contrastively by means of duration and fundamental frequency (F0) with an auditory stimuli of another EEG experiment, which has the same research questions without an acoustic preprocessing. Zero-crossing (0-) of the auditory stimuli are determined by using fundamental frequency and duration and the recordings keep their naturality. This preliminary method on Turkish provides more reliable results with the use of auditory stimuli that was built on brain studies in which the segmental and suprasegmental phonologic components in Turkish are investigated. Findings of this study reveals the importance of acoustic preprocessing in Turkish by examining the auditory stimuli with statistical methods. According to these findings, the way this method affects the results of the experiment and the necessity of using an acoustic preprocessing in two different brain imaging researches which try to answer the same linguistic issues are emphasized for future studies.