RNA-Seq Profiling of Circular RNAs and the Oncogenic Role of circPVT1 in Cutaneous Squamous Cell Carcinoma

Abstract

Shuang Chen, Junli Ding, Yunlin Wang, Tao Lu, Lili Wang, Xinghua Gao, Hongduo Chen, Le Qu, Chundi He Department of Dermatology, No.1 Hospital of China Medical University, Hospital of China Medical University, Key Laboratory of Immunohematology, Shenyang, Liaoning, People’s Republic of ChinaCorrespondence: Chundi He; Le QuDepartment of Dermatology, No.1 Hospital of China Medical University, Key Laboratory of Immunodermatology, 155 North Nanjing Street, Shenyang, Liaoning 110001, People’s Republic of ChinaTel/ Fax [phone]Email [email protected]; [email protected]: Cutaneous squamous cell carcinoma (CSCC) is associated with a poor 5-year survival rate. circRNAs have an important role in a number of physiological and pathological processes. However, the relationship between circRNAs and cutaneous squamous cell carcinoma (CSCC) is unclear.Purpose: The aim of the present study was to investigate the expression of circRNAs in cutaneous squamous cell carcinoma (CSCC) and its effect on CSCC proliferation and metastasis.Methods: We used high-throughput sequencing (RNA-seq) to identify circRNAs that were differentially in CSCC tissue and their paracarcinoma tissue. Quantitative real-time PCR results confirm deep-sequencing findings in CSCC tissue and cell lines. CCK-8 assay and flow cytometry were used to detect the effect of circPVT1 on the proliferation and migration of CSCC cells.Results: We identified 449 circRNAs that were differentially expressed between CSCC and normal adjacent tissue samples. circPVT1 (hsa_circ_0001821) was further researched to confirm its oncogene role in CSCC.Conclusion: Differentially expressed circular RNA plays an important role in the development of CSCC, and circPVT1 may be an important target for the treatment of CSCC.Keywords: circRNA, RNA-seq, CSCC, circPVT1

Keywords

• circrna
• rna-seq
• cscc
• circpvt1