International Journal of Nanomedicine (Nov 2019)
Ta-Coated Titanium Surface With Superior Bacteriostasis And Osseointegration
Abstract
Xiao-Meng Zhang,* Yuan Li,* Ying-Xin Gu, Chu-Nan Zhang, Hong-Chang Lai, Jun-Yu Shi Department of Implant Dentistry, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hong-Chang Lai; Jun-Yu ShiDepartment of Implant Dentistry, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 500 Quxi Road, Shanghai 200011, People’s Republic of ChinaTel +86 21 23271699 (ext. 5298)Fax +86 21 53073068Email [email protected]; [email protected]: Although tantalum (Ta)-based coatings have been proven to have good antibacterial activity, the underlying mechanism and in vivo biological performance remain unclear, which are essential for the clinical application of Ta-coated biomaterials as dental implants.Purpose: The main objective of this study is to investigate the antibacterial activity of Ta-modified titanium (Ti) implants against peri-implantitis-related microbes and the potential molecular mechanisms.Methods: Fusobacterium nucleatum and Porphyromonas gingivalis were selected to evaluate the antibacterial activity and potential antibacterial mechanism of Ta modification. The in vivo biocompatibility of Ta-modified implants was also evaluated.Results: The results showed that Ta-modified surface performed excellent antimicrobial activity against Fusobacterium nucleatum and Porphyromonas gingivalis. Micro galvanic might be formed between the incorporated Ta and the Ti base, which could consume the protons and result in decreased ATP synthesis and increased ROS generation. The gene expression of bacterial virulence factors associated with cellular attachment, invasion and viability as the target of ROS was downregulated. Importantly, in vivo biological studies showed that Ta modification significantly promoted the osseointegration of implants by stimulating the expression of bone-forming proteins.Conclusion: This study may provide some insights into clinical applications of Ta-coated Ti implants, especially in possibly infected situations.Keywords: tantalum-coating, antibacterial activity, ATP synthesis, ROS generation, osseointegration
