Amikacin sulfate loaded natural rubber latex films: Characterization and antibacterial performances

Honey Gopinathan; Jiya Jose; Anmiya Peter; Chandini C. Mohan; Harishma P. C; Sunil K. Narayanankutty; Sarita G Bhat

Results in Engineering (Sep 2024)

Amikacin sulfate loaded natural rubber latex films: Characterization and antibacterial performances

Honey Gopinathan,
Jiya Jose,
Anmiya Peter,
Chandini C. Mohan,
Harishma P. C,
Sunil K. Narayanankutty,
Sarita G Bhat

Affiliations

Honey Gopinathan: Department of Biotechnology, Cochin University of Science and Technology, Kochi, 682022, Kerala, India; Department of Polymer Science and Rubber Technology, Cochin University of Science and Technology, Kochi, 682022, Kerala, India
Jiya Jose: Department of Biotechnology, Cochin University of Science and Technology, Kochi, 682022, Kerala, India; Division of Microbiology, Department of Biosciences, Rajagiri College of Social Sciences (Autonomous), Kalamassery, Kochi, India
Anmiya Peter: Department of Biotechnology, Cochin University of Science and Technology, Kochi, 682022, Kerala, India; Department of Polymer Science and Rubber Technology, Cochin University of Science and Technology, Kochi, 682022, Kerala, India
Chandini C. Mohan: Department of Biotechnology, Cochin University of Science and Technology, Kochi, 682022, Kerala, India
Harishma P. C: Department of Biotechnology, Cochin University of Science and Technology, Kochi, 682022, Kerala, India
Sunil K. Narayanankutty: Department of Polymer Science and Rubber Technology, Cochin University of Science and Technology, Kochi, 682022, Kerala, India
Sarita G Bhat: Department of Biotechnology, Cochin University of Science and Technology, Kochi, 682022, Kerala, India; Inter University Centre for Nanomaterials and Devices (IUCND), Cochin University of Science and Technology, Kerala, 682022, India; Corresponding author. Department of Biotechnology, Cochin University of Science & Technology, Kochi, 682022, Kerala, India.

Journal volume & issue: Vol. 23
p. 102795

Abstract

Read online

Natural rubber latex (NRL), obtained from Hevea brasiliensis, comprises particles of long-chain poly(cis-1,4-isoprene) molecules. It is a naturally occurring, biocompatible polymer that acts as a stable matrix for precise drug release. Amikacin sulfate (AS) is a broad-spectrum semi-synthetic aminoglycoside antibiotic frequently used to treat Gram-negative bacterial infections. This study focuses on developing amikacin sulfate-loaded NRL (AS-NRL) films, characterizing their physicochemical properties, evaluating their antibacterial and antibiofilm activities, and assessing their cytocompatibility. The AS-NRL films were prepared using a casting method. Their physicochemical properties were characterized by fourier transform infrared spectroscopy (FTIR), mechanical testing, scanning electron microscopy (SEM), and static contact angle assessments. The antibacterial and antibiofilm efficacy of the films was evaluated against strong biofilm-forming, multi-drug resistant (MDR) strains of Klebsiella spp. and Pseudomonas spp., isolated from indwelling urinary catheters (IUCs). This investigation confirmed that amikacin sulfate was effectively incorporated into the natural rubber latex films. Surface topography analysis by SEM also confirmed the presence of amikacin sulfate on the film surface. All films possessed sufficient elasticity for catheterization. The antibacterial and antibiofilm assays confirmed the ability of the AS-NRL films to control bacterial growth and minimize biofilm formation, supported by live/dead staining assays and SEM imaging. NRLA4 films retained antibacterial activity after leaching in artificial urine for 21 days against all tested bacterial strains. The AS-NRL films showed cytocompatibility in vitro. This work demonstrates that AS-NRL films are promising biomaterials for developing urinary catheters, potentially reducing CAUTI and the undesirable side effects of high-dose antibiotics.

Published in Results in Engineering

ISSN: 2590-1230 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology
Website: https://www.journals.elsevier.com/results-in-engineering

About the journal

Abstract

Keywords