Effect of Supplementation with Coffee and Cocoa By-Products to Ameliorate Metabolic Syndrome Alterations Induced by High-Fat Diet in Female Mice
Cheyenne Braojos,
Andrea Gila-Díaz,
Pilar Rodríguez-Rodríguez,
Ignacio Monedero-Cobeta,
María Dolores Morales,
Santiago Ruvira,
David Ramiro-Cortijo,
Vanesa Benítez,
María A. Martín-Cabrejas,
Silvia M. Arribas
Affiliations
Cheyenne Braojos
Institute of Food Science Research (CIAL), Universidad Autónoma de Madrid (UAM-CSIC), C/Nicolás Cabrera 9, 28049 Madrid, Spain
Andrea Gila-Díaz
Food, Oxidative Stress and Cardiovascular Health (FOSCH) Research Group, Universidad Autónoma de Madrid (UAM-CSIC), Ciudad Universitaria de Cantoblanco, 28049 Madrid, Spain
Pilar Rodríguez-Rodríguez
Food, Oxidative Stress and Cardiovascular Health (FOSCH) Research Group, Universidad Autónoma de Madrid (UAM-CSIC), Ciudad Universitaria de Cantoblanco, 28049 Madrid, Spain
Ignacio Monedero-Cobeta
Food, Oxidative Stress and Cardiovascular Health (FOSCH) Research Group, Universidad Autónoma de Madrid (UAM-CSIC), Ciudad Universitaria de Cantoblanco, 28049 Madrid, Spain
María Dolores Morales
Confocal Microscopy Unit, Interdepartmental Research Service (SiDI), Faculty of Medicine, Universidad Autónoma de Madrid (UAM-CSIC), C/Arzobispo Morcillo 2, 28029 Madrid, Spain
Santiago Ruvira
Food, Oxidative Stress and Cardiovascular Health (FOSCH) Research Group, Universidad Autónoma de Madrid (UAM-CSIC), Ciudad Universitaria de Cantoblanco, 28049 Madrid, Spain
David Ramiro-Cortijo
Food, Oxidative Stress and Cardiovascular Health (FOSCH) Research Group, Universidad Autónoma de Madrid (UAM-CSIC), Ciudad Universitaria de Cantoblanco, 28049 Madrid, Spain
Vanesa Benítez
Institute of Food Science Research (CIAL), Universidad Autónoma de Madrid (UAM-CSIC), C/Nicolás Cabrera 9, 28049 Madrid, Spain
María A. Martín-Cabrejas
Institute of Food Science Research (CIAL), Universidad Autónoma de Madrid (UAM-CSIC), C/Nicolás Cabrera 9, 28049 Madrid, Spain
Silvia M. Arribas
Food, Oxidative Stress and Cardiovascular Health (FOSCH) Research Group, Universidad Autónoma de Madrid (UAM-CSIC), Ciudad Universitaria de Cantoblanco, 28049 Madrid, Spain
Coffee and cocoa manufacturing produces large amounts of waste. Generated by-products contain bioactive compounds with antioxidant and anti-inflammatory properties, suitable for treating metabolic syndrome (MetS). We aimed to compare the efficacy of aqueous extracts and flours from coffee pulp (CfPulp-E, CfPulp-F) and cocoa shell (CcShell-E, CcShell-F) to ameliorate MetS alterations induced by a high-fat diet (HFD). Bioactive component content was assessed by HPLC/MS. C57BL/6 female mice were fed for 6 weeks with HFD followed by 6 weeks with HFD plus supplementation with one of the ingredients (500 mg/kg/day, 5 days/week), and compared to non-supplemented HFD and Control group fed with regular chow. Body weight, adipocyte size and browning (Mitotracker, confocal microscopy), plasma glycemia (basal, glucose tolerance test–area under the curve, GTT-AUC), lipid profile, and leptin were compared between groups. Cocoa shell ingredients had mainly caffeine, theobromine, protocatechuic acid, and flavan-3-ols. Coffee pulp showed a high content in caffeine, protocatechuic, and chlorogenic acids. Compared to Control mice, HFD group showed alterations in all parameters. Compared to HFD, CcShell-F significantly reduced adipocyte size, increased browning and high-density lipoprotein cholesterol (HDL), and normalized basal glycemia, while CcShell-E only increased HDL. Both coffee pulp ingredients normalized adipocyte size, basal glycemia, and GTT-AUC. Additionally, CfPulp-E improved hyperleptinemia, reduced triglycerides, and slowed weight gain, and CfPulp-F increased HDL. In conclusion, coffee pulp ingredients showed a better efficacy against MetS, likely due to the synergic effect of caffeine, protocatechuic, and chlorogenic acids. Since coffee pulp is already approved as a food ingredient, this by-product could be used in humans to treat obesity-related MetS alterations.
