Immunohistochemical Expression of Xenophagy Proteins in Helicobacter pylori and None Helicobacter pylori Gastritis

Abstract

The complex interplay between H. pylori and autophagy was elucidated in gastritis in term of host defense and survival of microbe. However, H. pylori frequently succeed in their survival and develops more aggressive tissue damage by different ways. A total of 80 gastritis patients undergoing gastroscopy during September, 2013 to August, 2014. Formalin fixed paraffin embedded gastric tissue were prepared for immunohistochemistry evaluation of ATG16L1, LC3C and vacA protein expression. Rapid urease test was used to discriminate between H. pylori or none H. pylori gastritis. Xenophagy index was elevated among both urease and IgG positive cases (29.86% and 27.13%) compared with negative cases (13.43% and 17.6% respectively (p<0.001). vacA have been found in 62.5% of urease positive cases and 55.88% of IgG positive cases. The higher xenophagy index score found to be associated with vacA positive cases (p=0.002). Xenophagy index to be associated with chronic H. pylori infection and this might be related to vacA positivity.

Keywords

• h. pylori
• xenophagy
• gastritis