Biotechnologia Acta (Jun 2024)

PHOTOREGULATION OF THE BIOSYNTHETIC ACTIVITY OF LARICIFOMES OFFICINALIS USING COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION

  • O.B. Mykhaylova,
  • A.M. Negriyko,
  • K.G. Lopatko ,
  • N. Shchotkina,
  • N.L. Poyedinok

DOI
https://doi.org/10.15407/biotech17.03.066
Journal volume & issue
Vol. 17, no. 3
pp. 66 – 77

Abstract

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The aim of the work was to study the influence of biogenic metal nanoparticles on the growth characteristics and biosynthetic activity of the fungus Laricifomes officinalis, as well as the effects of photocatalytic activity of NPs after exposure to low-intensity laser radiation under deep cultivation conditions. Material and Methods. Traditional mycological methods, colloidal solutions of nanoparticles biogenic metals, and unique photobiological methods were used. The effect of light on the biosynthetic acti vity of L. officinalis was studied using low-intensity coherent monochromatic laser light with specified spectral and intensity characteristics. The experiment used water-based colloidal solutions of biogenic metals such as Fe, Mg and Ag, obtained by a patented method. Results. Treatment of the inoculum with colloidal solutions of nanoparticles of all used metals ncreased the growth of L. officinalis by 31–54%, while irradiation of the fungal inoculum with laser light in a medium with nanoparticles reduced the growth activity of the L. officinalis mycelium by 14.4–22.6%. All nanoparticles suppressed the biosynthesis of extracellular polysaccharides, whereas treatment of the inoculum with colloidal solutions of FeNPs and MgNPs stimulated the synthesis of endopolysaccharides. At the same time, laser light irradiation in the presence of AgNPs increased the amount of endopolysaccharides, while FeNPs and MgNPs slightly inhibited their synthesis. Treatment of the inoculum with colloidal metal solutions and laser light affected the total phenolic content (TPC) in the mycelial mass. The highest TPC values in ethanol extracts with AgNPs and laser light irradiation were 97.31±3.7 mg of GAEs/g of dry mass. Conclusions. The research results gave ground to consider nanoparticles of biogenic metals (AgNPs, FeNPs, MgNPs) and low-intensity laser light as a promising regulators of the biosynthetic activity of L. officinalis in the biotechnology of its cultivation.

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