Influence of light intensity and photoperiod on the pigment and, lipid production of Dunaliella tertiolecta and Nannochloropsis oculata under three different culture medium

Abstract

Microalgal biomass has the ability to store huge amount of triacylglycerides as fatty ester methyl esters (FAME) and carotenoids which has made algae as potential candidate for biorefinery approach. Essential fatty acid such as palmitic acid, stearic acid, arachidonic acid and eicospentanoic acid have been produced which are known for their various applications. The present study was aimed to evaluate the influence of different light intensities (120 and 250 μE/m2/s) and photoperiod (16:8h and 13:11h light/dark cycle) on the production of lipid, biomass and lutein. Dunaliella tertiolecta and Nannochloropsis oculata was grown for 23 days in F/2, sea salt media (SSM, Distilled water (DW) and SSM (natural seawater media,NSW) under two different light intensities and photoperiod regimes at 25 ᵒC. SSM (NSW) showed maximum accumulation of lipid in D.tertiolecta (34.56 mg/L/d). SSM (DW)- biomass showed 1.5 times higher lutein productivity of 0.253 mg/L/d under 13:11h light/dark cycle at 250 μE/m2/s compared to same medium under 16:8h light/dark cycles at 120 μE/m2/s. Where as in N.oculata, F/2 biomass showed higher lipid and lutein productivity of 15.69 and 0.279 mg/L/d, respectively The laboratory scale cultivation parameters and related media cost showed the suitability of different culture media adaptation to large scale production.

Keywords

• Microalgae
• Light intensity
• Photoperiod
• Lipid
• Lutein