Determination of Sennosides A and B in Sudanese Medicinal Plant Senna alexandrina Pods and Leaves using Reverse-Phase High Performance Liquid Chromatography

Abstract

Senna alexandrina pods and leaves have been used in herbal medicine since ancient times. The extracts of this plant contain mainly anthraquinone glycosides that have a significant laxative effect. In this study, the sennosides A and B, the major constituents of Senna alexandrina were calculated via a simple and accurate HPLC method. The yield of sennoside A was found 1.22 and 1.83% in the pods and leaves, respectively. Whereas the yield of sennoside B was found 2.7 and 1.83% in the pods and leaves, respectively.

Keywords

• senna alexandrina
• anthraquinone glycosides
• sennosides
• laxative effect