Structure and activity of particulate methane monooxygenase arrays in methanotrophs

Yanan Zhu; Christopher W. Koo; C. Keith Cassidy; Matthew C. Spink; Tao Ni; Laura C. Zanetti-Domingues; Benji Bateman; Marisa L. Martin-Fernandez; Juan Shen; Yuewen Sheng; Yun Song; Zhengyi Yang; Amy C. Rosenzweig; Peijun Zhang

doi:10.1038/s41467-022-32752-9

Nature Communications (Sep 2022)

Structure and activity of particulate methane monooxygenase arrays in methanotrophs

Yanan Zhu,
Christopher W. Koo,
C. Keith Cassidy,
Matthew C. Spink,
Tao Ni,
Laura C. Zanetti-Domingues,
Benji Bateman,
Marisa L. Martin-Fernandez,
Juan Shen,
Yuewen Sheng,
Yun Song,
Zhengyi Yang,
Amy C. Rosenzweig,
Peijun Zhang

Affiliations

Yanan Zhu: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
Christopher W. Koo: Departments of Molecular Biosciences and of Chemistry, Northwestern University
C. Keith Cassidy: Department of Biochemistry, University of Oxford
Matthew C. Spink: Diamond Light Source, Harwell Science and Innovation Campus
Tao Ni: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
Laura C. Zanetti-Domingues: Central Laser Facility, Science and Technology Facility Council, Rutherford Appleton Laboratory
Benji Bateman: Central Laser Facility, Science and Technology Facility Council, Rutherford Appleton Laboratory
Marisa L. Martin-Fernandez: Central Laser Facility, Science and Technology Facility Council, Rutherford Appleton Laboratory
Juan Shen: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
Yuewen Sheng: Diamond Light Source, Harwell Science and Innovation Campus
Yun Song: Diamond Light Source, Harwell Science and Innovation Campus
Zhengyi Yang: Diamond Light Source, Harwell Science and Innovation Campus
Amy C. Rosenzweig: Departments of Molecular Biosciences and of Chemistry, Northwestern University
Peijun Zhang: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford

DOI: https://doi.org/10.1038/s41467-022-32752-9
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

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Particulate methane monooxygenase (pMMO) is the main enzyme used by methanotrophs. Here, the authors determined the native structure of pMMO by cryo-electron tomography, revealing lipid-stabilized features and a higher-order hexagonal array arrangement in intact cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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