Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections

Abstract

Summary: Measurements of telomere length in skeletal muscle stem cells (MuSCs), a rare cell population within muscles, provide insights into cellular dysfunction in diseased conditions. Here, we describe a protocol (cryosection muscle quantitative fluorescent in situ hybridization) using skeletal muscle cryosections for assessments of telomere length in MuSCs, in their native environment. Using a free software, telomere length measurements are assessed on a single-cell level. We also provide methodology to perform data analyses in several ways.For complete details on the use and execution of this protocol, please refer to Tichy et al. (2021).

Keywords

• Cell Biology
• Cell isolation
• Cell-based Assays
• In Situ Hybridization
• Microscopy
• Stem Cells